Application of Biotechnology for the Production of New Food Preservations
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Abstract
The presence of pathogenic fungi has been a global concern has economical affects. Recently prevent the fungal growth using biological control using halal source become an important strategy for limiting mycotoxigenic fungi. Therefore the aim of this study that the use of some LAB bacteria and screen their antimicrobial activity against pathogenic fungi. Halal bacteria extracts were studied for the antimicrobial activity. The results indicated that two isolates were identified. Meanwhile a total of 90 fungal isolates were isolated from halal food samples. Aspergillus was the dominant fungi (65%) in five species namely, parasiticus, flavus ochraceus, terreus, and niger. Fusarium (clomorum and graminerum) and Penicillium were in lower frequency. Halal bacterial extracts were reduced the mycelium dry weight of all tested fungi and showed clear zone inhibition. Molecular identification of mycotoxin producing fungi was estimated. In conclusion, the study revealed the probability of using halal Enterococcus isolates in food preservation to ensure halal food safety. The partial or total sequencing of the 16s ribosomal DNA (rRNA) gene showed a fast technique used fungi classification
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S. E.-S. Aly and N. A. Emam, “Application of Biotechnology for the Production of New Food Preservations”, Int.J.Halal.Res, vol. 2, no. 1, pp. 16-29, Jun. 2020.
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References
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Abdulateef, S.M., Aljubori, M.H., Abdulbaqi, N.J., 2014. Genetic diversity among some Aspergillus flavus isolates by using Inter-simple sequence repeats (ISSR). Iraqi J. Sci. 55(3), 986-993.
Adimpong, D.B., Nielsen, D.S., Sørensen, K.I., Derkx, P.M., Jespersen, L., 2012. Genotypic characterization and safety assessment of lactic acid bacteria from indigenous African fermented food products. BMC Microbiol. 12. 10.1186/1471-2180-12-75.
Adss, A.A., Abdel-Gayed, M.A., Botros, W., Hafez, E.E., 2017. Multilocus genetic techniques, RAPD-PCR and ISSR-PCR Markers and polygalacturonase activity as tools for differentiation among alternaria solani isolates on tomato fruits and relation to their pathogenicity in Egypt. Asian J. Plant Pathol. 11(1), 18-27.
Aly, S.E., Abo-Sereih, N.A., Salim, R.G., Hathout, A.S., 2018. Isolation and molecular identification of food grade lactic acid bacteria and their antifungal activity. J. Biolo. Sci. 18(6), 260-269.
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Felis, G. E., & Dellaglio, F. (2007). Taxonomy of Lactobacilli and Bifidobacteria. Current Issues Intestinal Microbiology, 8(2), 44-61.
Fonseca, H., 2012. A aflatoxina e o amendoim. Boletim Técnico no. 13 [Online]. http://www.micotoxinas.com.br/Boletim13.htm.
Giraffa, G., 2003. Functionality of enterococci in dairy products. Int. J. Food Microbiol. 88(2-3), 215-222.
Hathout, A.S., Aly, S.E., 2010. Role of lactic acid bacteria as a biopreservative agent of Talbina. J. Am. Sci. 6, 889-898.
Hathout, A.S., Aly, S.E., 2014. Biological detoxification of mycotoxins: a review. Ann. Mocrobiol. 64(3), 905-919.
Henry, T., Iwen, P.C., Hinrichs, S.H., 2000. Identification of Aspergillus species using internal transcribed spacer regions 1 and 2. J. Clin. Microbiol. 38, 1510-1515.
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Lahtinen, C.A., Haskard, A.C., Ouwehand, S.J., Salminen, J., Ahokas, T., 2004. Binding of aflatoxin B1 to cell wall components of Lactobacillus rhamnosus strain GG. Food Add. Contam. 21, 158-164.
Lavanya, R., Sowmiya, S., Balaji, S., Muthuvelan, B., 2011. Screening and characterization of lactic acid bacteria from fermented milk. Brit. J. Dairy Sci. 2(1), 5-10.
Leslie, J.F., Summerell, B.A., 2006. The Fusarium laboratory manual. UK: Blackwell Publishing Ltd.
Magnoli, C, Astoreca, A., Ponsone, M.L., Fernández-Juri, M.J., Barberis, C., Dalcero, A.M., 2007. Ochratoxin A and Aspergillus section Nigri in peanut seeds at different months of storage in Córdoba, Argentina. Int. J. Food Microbiol. 119(3), 213-218.
Mahmoud, M.A., EL-Samawaty, A.M.A., Yassin, M.A., Abd EL-Aziz, A.R.M., 2016. Genetic diversity analysis of Aspergillus flavus isolates from plants and air by ISSR Markers. Gen. Mol. Res. 15(2), 15028.
Marcos Filho, J., 2005. Fisiologia de sementes de plantas cultivadas. Piracicaba: FEALQ.495.
Nakai, V.K., Rocha, L.O., Gonçalez, E., Fonseca, H., Ortega, E.M.M., Corrêa, B., 2008. Distribution of fungi and aflatoxins in a stored peanut variety. Food Chem. 106, 285-290.
Nelson, P.E., Toussoun, T.A., Marasas, W.F.O.., 1983. Fusarium species. An illustrated manual for identification. University Park: Pennsylvania State University Press. 193.
Niderkorn, V., Boudra, H., Morgavi, D.P., 2006. Binding of Fusarium mycotoxins by fermentative bacteria in vitro. J. Appl. Microbiol. 101,849-856.
Pildain, M.B., Frisvad, J.C., Vaamonde, G., Cabral, D., Varga, J., Samson, R.A., 2008. Two novel aflatoxin-producing Aspergillus species from Argentinian peanuts. Int. J. Syst. Evolut. Microbiol. 58,725-735.
Rohlf, F.J., 1993. NTSYS-pc: Em unumerical taxonomy and multivariate system. Version 2.9. New York. Applied Biostatistics. Schimmelcuhves, pp: 116. The Netherlands species using internal transcribed spacer regions 1 and 2. J. Clin. Microbiol. 9, 213.
Sabry, B.A., Hathout A.S., Nooh, A., Aly, S.E., Shehata, M., 2016. The prevalenc of aflatoxin and Aspergillus parasiticus in Egyptian sesame seeds. Int. J. ChemTech Rese. 9(11), 308-319.
Sahab, A.F., Aly, S.E., Nawar, L.S., El-Faham, S.Y., 2011. Fungal occurrence in physic nut (Jatropha curcas) seeds during storage and possibility aflatoxin production by Aspergillus flavus and Aspergillus parasiticus isolates. J. Am. Sci. 21, 342
Sahab, A.F., Hathout, A.S., Sabry, B.A., Aly, S.E., 2014. Application of some plant essential oils to control Fusarium isolates associated with freshly harvested maize in Egypt. J. Essen. Oil Bear. Plants 17(6), 1146 -1155.
Targowski, T., Płusa, T., 2015. Overall principles of treatment in case of 689 toxicological threats. Polski Merkuriusz Lekarski 39 (231), 193-198.
Větrovský, T., Baldrian, P., Neufeld, J., 2013.The variability of the16S rRNA gene in bacterial genomes and its consequences for bacterial community analyses. Plos One 8(2), e57923 doi: 10.1371/journal.pone.0057923.
Vignolo, G., Castellano, P., Belfiore, C., Fadda, S., 2008. A review of bacteriocinogenic lactic acid bacteria used as bio-protective cultures in fresh meat produced in Argentina. Meat Sci. 79(3), 483-99. doi: 10.1016/j.meatsci.2007.10.009.
White, T.J., Bruns, T., Lee, S., Taylor, J.W., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications (Innis MA, Gelfand DH, Sninsky JJ and White TJ, eds.). Academic Press Inc., New York, 315-322.
Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey S.V., 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 6531-6535.
Yugander, A., Ladhalakshmi, D., Prakasham, V., Mangrauthia, S.K., Prasad, M.S., Madhav, M.S., Sundaram, R.M., Laha, G.S., 2015. Pathogenic and genetic variation among the isolates of Rhizoctonia solani (AG 1-IA), the Rice sheath blight pathogen. J. Phytopathol. 163(6), 465-474.
Abdel-Shafi, S., Al-Mohammadi, A.R., Negm, S., Enan, G., 2014. Antibacterial activity of Lactobacillus delbreukiisub species bulgaricus isolated from Zabady, Life Sci. J. 11, 264-270.
Abdel-Wahhab, M.A., Hassan, N.S., El-Kady, A.A., Khadrawy, Y.A., El-Nekeety, A.A., Mohamed, S.R., Sharaf, H.A., Mannaa, F.A., 2010. Red ginseng extract protects against aflatoxin B1 and fumonisins-induced hepatic pre-cancerous lesions in rats. Food Chem. Toxicol. 48(2), 733-742.
Abdulateef, S.M., Aljubori, M.H., Abdulbaqi, N.J., 2014. Genetic diversity among some Aspergillus flavus isolates by using Inter-simple sequence repeats (ISSR). Iraqi J. Sci. 55(3), 986-993.
Adimpong, D.B., Nielsen, D.S., Sørensen, K.I., Derkx, P.M., Jespersen, L., 2012. Genotypic characterization and safety assessment of lactic acid bacteria from indigenous African fermented food products. BMC Microbiol. 12. 10.1186/1471-2180-12-75.
Adss, A.A., Abdel-Gayed, M.A., Botros, W., Hafez, E.E., 2017. Multilocus genetic techniques, RAPD-PCR and ISSR-PCR Markers and polygalacturonase activity as tools for differentiation among alternaria solani isolates on tomato fruits and relation to their pathogenicity in Egypt. Asian J. Plant Pathol. 11(1), 18-27.
Aly, S.E., Abo-Sereih, N.A., Salim, R.G., Hathout, A.S., 2018. Isolation and molecular identification of food grade lactic acid bacteria and their antifungal activity. J. Biolo. Sci. 18(6), 260-269.
Barnett, H.L., Hunter, B.B., 1986. Illustrated genera of fungi. 3rd ed. Minneapolis, MN: Burgess Co.
Bintvihok, A., Thiengnin, S., Doi, K., Kumagai, S., 2002. Residues of aflatoxins in the liver, muscle and eggs of domestic fowls. J. Vet. Med. Sci. 64, 1037-1039.
Chadha, S., Gopalakrishna, T., 2007. Comparative assessment of REMAP and ISSR marker assays for genetic polymorphism studies in Magnaporthe grisea. Curr. Sci. 93, 688-692.
Felis, G. E., & Dellaglio, F. (2007). Taxonomy of Lactobacilli and Bifidobacteria. Current Issues Intestinal Microbiology, 8(2), 44-61.
Fonseca, H., 2012. A aflatoxina e o amendoim. Boletim Técnico no. 13 [Online]. http://www.micotoxinas.com.br/Boletim13.htm.
Giraffa, G., 2003. Functionality of enterococci in dairy products. Int. J. Food Microbiol. 88(2-3), 215-222.
Hathout, A.S., Aly, S.E., 2010. Role of lactic acid bacteria as a biopreservative agent of Talbina. J. Am. Sci. 6, 889-898.
Hathout, A.S., Aly, S.E., 2014. Biological detoxification of mycotoxins: a review. Ann. Mocrobiol. 64(3), 905-919.
Henry, T., Iwen, P.C., Hinrichs, S.H., 2000. Identification of Aspergillus species using internal transcribed spacer regions 1 and 2. J. Clin. Microbiol. 38, 1510-1515.
Iqbal, S.Z., Asi, M.R., Zuber, M., Akhtar, J., Saif, M.J., 2013. Natural occurrence of aflatoxins and ochratoxin A in commercial chilli and chilli sauce samples. Food Cont. 30(2), 621-625.
Khan, H., Flint, S., Yu, P.L., 2010. Enterocins in food preservation. Int. J.Food Microbiol. 141(1- 2), 1-10.
Lahtinen, C.A., Haskard, A.C., Ouwehand, S.J., Salminen, J., Ahokas, T., 2004. Binding of aflatoxin B1 to cell wall components of Lactobacillus rhamnosus strain GG. Food Add. Contam. 21, 158-164.
Lavanya, R., Sowmiya, S., Balaji, S., Muthuvelan, B., 2011. Screening and characterization of lactic acid bacteria from fermented milk. Brit. J. Dairy Sci. 2(1), 5-10.
Leslie, J.F., Summerell, B.A., 2006. The Fusarium laboratory manual. UK: Blackwell Publishing Ltd.
Magnoli, C, Astoreca, A., Ponsone, M.L., Fernández-Juri, M.J., Barberis, C., Dalcero, A.M., 2007. Ochratoxin A and Aspergillus section Nigri in peanut seeds at different months of storage in Córdoba, Argentina. Int. J. Food Microbiol. 119(3), 213-218.
Mahmoud, M.A., EL-Samawaty, A.M.A., Yassin, M.A., Abd EL-Aziz, A.R.M., 2016. Genetic diversity analysis of Aspergillus flavus isolates from plants and air by ISSR Markers. Gen. Mol. Res. 15(2), 15028.
Marcos Filho, J., 2005. Fisiologia de sementes de plantas cultivadas. Piracicaba: FEALQ.495.
Nakai, V.K., Rocha, L.O., Gonçalez, E., Fonseca, H., Ortega, E.M.M., Corrêa, B., 2008. Distribution of fungi and aflatoxins in a stored peanut variety. Food Chem. 106, 285-290.
Nelson, P.E., Toussoun, T.A., Marasas, W.F.O.., 1983. Fusarium species. An illustrated manual for identification. University Park: Pennsylvania State University Press. 193.
Niderkorn, V., Boudra, H., Morgavi, D.P., 2006. Binding of Fusarium mycotoxins by fermentative bacteria in vitro. J. Appl. Microbiol. 101,849-856.
Pildain, M.B., Frisvad, J.C., Vaamonde, G., Cabral, D., Varga, J., Samson, R.A., 2008. Two novel aflatoxin-producing Aspergillus species from Argentinian peanuts. Int. J. Syst. Evolut. Microbiol. 58,725-735.
Rohlf, F.J., 1993. NTSYS-pc: Em unumerical taxonomy and multivariate system. Version 2.9. New York. Applied Biostatistics. Schimmelcuhves, pp: 116. The Netherlands species using internal transcribed spacer regions 1 and 2. J. Clin. Microbiol. 9, 213.
Sabry, B.A., Hathout A.S., Nooh, A., Aly, S.E., Shehata, M., 2016. The prevalenc of aflatoxin and Aspergillus parasiticus in Egyptian sesame seeds. Int. J. ChemTech Rese. 9(11), 308-319.
Sahab, A.F., Aly, S.E., Nawar, L.S., El-Faham, S.Y., 2011. Fungal occurrence in physic nut (Jatropha curcas) seeds during storage and possibility aflatoxin production by Aspergillus flavus and Aspergillus parasiticus isolates. J. Am. Sci. 21, 342
Sahab, A.F., Hathout, A.S., Sabry, B.A., Aly, S.E., 2014. Application of some plant essential oils to control Fusarium isolates associated with freshly harvested maize in Egypt. J. Essen. Oil Bear. Plants 17(6), 1146 -1155.
Targowski, T., Płusa, T., 2015. Overall principles of treatment in case of 689 toxicological threats. Polski Merkuriusz Lekarski 39 (231), 193-198.
Větrovský, T., Baldrian, P., Neufeld, J., 2013.The variability of the16S rRNA gene in bacterial genomes and its consequences for bacterial community analyses. Plos One 8(2), e57923 doi: 10.1371/journal.pone.0057923.
Vignolo, G., Castellano, P., Belfiore, C., Fadda, S., 2008. A review of bacteriocinogenic lactic acid bacteria used as bio-protective cultures in fresh meat produced in Argentina. Meat Sci. 79(3), 483-99. doi: 10.1016/j.meatsci.2007.10.009.
White, T.J., Bruns, T., Lee, S., Taylor, J.W., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications (Innis MA, Gelfand DH, Sninsky JJ and White TJ, eds.). Academic Press Inc., New York, 315-322.
Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey S.V., 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 6531-6535.
Yugander, A., Ladhalakshmi, D., Prakasham, V., Mangrauthia, S.K., Prasad, M.S., Madhav, M.S., Sundaram, R.M., Laha, G.S., 2015. Pathogenic and genetic variation among the isolates of Rhizoctonia solani (AG 1-IA), the Rice sheath blight pathogen. J. Phytopathol. 163(6), 465-474.