Identification of Gelatin in Dental Materials using the Combination of Attenuated Total Reflection-Fourier Transform Infrared Spectroscopy (ATR-FTIR) and Chemometrics

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Published: Jun 30, 2020
DOI: https://doi.org/10.18517/ijhr.2.1.1-15.2020
Keywords:
gelatin, dental materials, ATR-FTIR, chemometric, PCA and SIMCA

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Dewi N. Mustaqimah
Faculty of Dentistry, Universitas YARSI, Jakarta, Indonesia
Anna P. Roswiem
Halal Research Center – Lembaga Penelitian Universitas YARSI, Jakarta, Indonesia

Abstract

Gelatin is a multifunctional ingredient widely used in the food and beverage industries. It is also useful in pharmaceutical industry, such as in the manufacturing of dental materials, whether as a main ingredient or an additive. The dental materials traded within Indonesia come from imported goods and local manufacturers. Gelatin is obtained through hydrolysis of cartilages, bones, and skins of cows, pigs and fish. Therefore, the presence of gelatin in dental materials posses critical haram risk for Moslems. This research aimed to examine the presence of gelatin in dental materials using Attenuated Total Reflection-Fourier Transform Infrared Spectroscopy (ATR-FTIR) combined with chemometrics. Gelatin was identified from comparing the infrared (IR) spectrum between samples and standard gelatin of bovine and porcine origins. We combined the IR data with chemometrics through Principal Component Analysis (PCA) and subsequent Soft Independent Modeling of Class Analogy (SIMCA) techniques in order to authenticate the gelatin source. The result showed that 4 out of 49 samples (DM-1, DM-6, DM-18, and DM-27) were detected with gelatin. Based on chemometrics analysis, one sample (DM-6) had similar profile with porcine gelatin. The research highlighted the potential use of combined techniques of ATR-FTIR and chemometrics in authenticating the gelatin source in pharmaceutical products.

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How to Cite
[1]
D. N. Mustaqimah and A. P. Roswiem, “Identification of Gelatin in Dental Materials using the Combination of Attenuated Total Reflection-Fourier Transform Infrared Spectroscopy (ATR-FTIR) and Chemometrics”, Int.J.Halal.Res, vol. 2, no. 1, pp. 1-15, Jun. 2020.
Issue
Vol 2 No 1 (2020)
Section
Articles
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References

Branden, K.V., & Hubert, M. (2005). Chemom. Intell. Lab. Syst., 79, 10-21.

Cole, B. (2000). Gelatin. New York. Jhon Wiley and Sons.

Demirhan, Y., Ulca, P. & Senyuva H.Z. (2012). Detection of porcine DNA in gelatin and gelatin containing processed food products-Halal/ kosher authentication. Meat. Sci., 90, 686-689.

Fischer, G., CaO, X., Cox, N., & Francis, M. (2005). The FTIR Spectra of glycine and glycyl-glycine. Zwitter ions isolated in alkali halide matrices. Journal of Chemical Physics, 313, 39-49.

Gelatin Food Science. (2007). Gelatin. http://www. Gelatin. Org. Cited January 18, 2013.

Glicksman, M. (1969). Gum Technolgy in Food Industry. New York, Academic Press.

GMIA. (2012). Gelatin Handbook. USA: Gelatin Manufactures Institute of America

Hashim, D.M., Che Man, Y.B., Norakasha, R., Suhaimi, M., Salmah, Y. & Shahariza, Z.A. (2010). Potential use of Fourier Trasform Infrared Spectroscopy for differentiation of bovine and porcine gelatins. Food Chemistry, 118(3), 856–860.

Hermanto, S., Sumarlin, L.O., & Fatimah, W. (2013). Differentiation of bovine and porcine gelatine based on spectroscopic and electrophoretic analysis. J. Food. Pharm. Sci., 1, 68-73.

Hidayatullah, F. 2014. Analisis Sumber Asal Gelatin Dalam Produk Pangan Komersial melalui Kombinasi metode Fourier Transform Infrared (FTIR) – Kemometri. Jakarta. Program Studi Kimia : Fakultas Sains dan Teknologi – UIN Syarif Hidayatullah Jakarta.

Irfanita, N., Jaswir I., Mirghani, M.E.S., Sukmasari, S., Ardini, Y.D. & Lestari, W. (2017). Rapid detection of gelatin in dental materials using attenuated total reflection fourier transform infrared spec-troscopy (ATR-FTIR). Journal of Physics: Conf. Series, 884 (1), 1-10.

Miller, J.N. & Miller, J.C. (2005). Statistics and Chemometrics for Analytical Chemistry. Fifth edition. Inggris: Pearson Education Limited.

Raraswati, M.A., Triyana, K. & Rohman, A. (2014). Differentiation of bovine and porcine gelatins in soft candy based on amino acid profiles and Chemometrics. J. Food. Pharm. Sci., 2, 1-6.

Rohman, A. & Che Man, Y.B. (2011). Analysis of Pig Derivatives for Halal Authentication Studies, food Revieew International, 28, 97-112.

Roswiem, A.P. 2015. Buku Saku Produk Halal Makanan dan Minuman. Jakarta. Republika.

Roswiem, A. P., Kesuma, I. 2018. Gelatin Indentification in Tablet Preparation of Drug using Fourier Transform Infrared Spetroscopy. Indonesian Journal of Halal. Vol. 1 (1), 59-73.

Rowe, R.C., Sherkey, P.J., & Quinn, M.E. (2009). Handbook of Phamaceutical Excipient. Sixth Edition, 2009. USA: Pharmaceutical Press and American Pharmacists Association.

Sahillah, A. M., Mohd. Fadly, L., Norrakiah, A.S., Aminah, A., Wan Aida, W.M., Ma’ruf, A.G., and Mohd. Khan, A. 2012. Halal Market Surveillance of Soft and Hard Gel Capsules in Pharmaceutical Product Using PCR and Biochip Analysis. Interntional Food and Research Journal. 19 (1) : 371-375

Syafiqoh, F. (2014). Analisis Gelatin Sapi dan Gelatin babi pada Produk Cangkang Kapsul Keras, Obat dan Vitamin menggunakan FTIR dan KCKT. Jakarta. Program Studi Farmasi : Fakultas Kedokteran dan Ilmu Kesehatan Universitas Islam Negeri Syarif Hidayatullah – Jakarta.

Tasara, T., Schumacher, S. & Stephan, R. (2015). Conventional and real-time PCR-based approaches for molecular detection and quantitation of bovine species material in edible gelatin. J. Food. Prod. 68, 2420-2426.