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Toothpaste products traded within Indonesia are produced locally and internationally. Some of the toothpaste products may contain gelatin, a protein derived from bovine or porcine skin and bones. There are only few researchers studying gelatin in the toothpaste. Therefore, this study aimed to examine the presence of gelatin in toothpaste using the Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy method combined with chemometrics. The presence of gelatin in the sample was identified from the similarity of the infrared spectrum profile between the sample and the standard gelatin of cow and pig origins. The spectra data sets from ATR-FTIR were subject to Principal Component Analysis (PCA) and subsequent Soft Independent Modeling of Class Analogy (SIMCA) method to precisely classify the origin of the gelatin. The study showed that the ATR-FTIR method can be used to identify the presence of gelatin in toothpaste products. Gelatin was detected in two out of 42 samples. However, the combination of the ATR-FTIR technique with chemometry could not provide excellent illustration of the origin of gelatin in the toothpaste products. We presumed that the gelatin was only added in small amounts in these samples to serve as a stabilising or emulsifying agent.
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Branden, K, V and Hubert., M., 2005 : Chemom. Intell. Lab. Syst. 79 : 10-21 (2005)
Cole, B. 2000. Gelatin. New York. Jhon Wiley and Sons
Demirhan, Y., Ulca, P. and Senyuva H. Z. 2012. Detection of porcine DNA in gelatin and gelatin containing processed food products-Halal/kosher authen-tication. Meat. Sci. 90, 686-9.
Fischer, G., CaO, X., Cox, N., & Francis, M. (2005). The FTIR Spectra of glycine and glycyl-glycine. Zwitter ions isolated in alkali halide matrices. Journal of Chemical Physics, 313, 39-49.
Gelatin Food Science. 2007 Gelatin. http : // www. Gelatin. Org (18 Januari. 2013)
Glicksman, M. 1969. Gum Technolgy in Food Industry. New York, Academic Press
GMIA, 2012. Gelatin Handbook. USA : Gelatin Manufactures Institute of Amerikca
Hashim., D.M., Che Man, Y.B., Norakasha, R., Shuhaimi, M., Shariza, Z.A. 2010. Potential Use of Fourier Trasform Infrared Spectroscopy for differentiation of bovine and porcine gelatins. Food Chemistry. 118 : 856 – 860.
Hermanto, S, Sumarlin, L. O. and Fatimah, W. 2013. Differentiation of bovine and pocine gelatine based on spectroscopic and electrophoretic analysis. J. Food. Pharm. Sci. 1 : 68-73.
Irfanita, et. al. 2017. Rapid detection of gelatin in dental materials using attenuated total reflection fourier transform infrared spectroscopy (ATR-FTIR). Journal of Physics : Conf. Series, 884 (2017) 012090.
Jaswir, I. 2010. Manajemen Analisis Produk Halal. ww. Foodreview. Biz (14 Januari 2013)
Miller, J. N., & Miller, J.C. 2005. Statistics and Chemometrics for Analytical Chemistry. Fifth edition. Inggris : Pearson Education Limited
Raraswati, M. A., Triyana, K. and Rohman, A. 2014 Differentiation of bovine and porcine gelatins in soft candy based on amino acid profiles and chemometrics. J. Food. Pharm. Sci. 2 1-6
Rohman, A. dan Che-man, Y. B. 2011. Analysis of Pig Derivatives for Halal Authentication Studies, food Revieew International, 28 : 97-112.
Rowe, R. C., Sherkey, P. J., and Quinn, M. E. 2009. Handbook of Phamaceutical Excipient. Sixth Edition, 2009. USA : Pharmaceutical Press and American Pharmcists Association.
Sahillah, A. M., et al. 2012. Halal Market Surveil lance of Soft and Hard Gel Capsules in Pharmaceutical Product Using PCR and Biochip Analysis. Interntional Food and Research Journal. 19 (1) : 371-375 (2012).
Srivastara, A. K, et al. 2011. Secondary Structure of Chorion Protein of the Lepidoptera Pericallia Ricini and Ariadne Merione by ATR FT-IR and Micro-Roman Spectroscopy. Int. J. Biol. Macromol 49, 317-22.
Tasara, T, Schumacher, S. and Stephan, R. 2015 Conventional and real-time PCR-based approaches for molecular detection and quantitation of bovine species material in edible gelatin. J. Food. Prod. 68 : 2420-6.
Vergoten, G., Theophanides, T. 2012, Biomolecular Structuree and Dynamics. Springer. Sci. Business. Media. 181-364.