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Nowadays L-Cysteine is widely used in the manufacture of food, drug and cosmetic products. In food industry, L-Cysteine usually used as a food additive in instant noodle which serves as a flavor enhancer for meat, in wheat flour and/or bakery products, it is functions as an improving agent and bread improver. On industrial scales, L-Cysteine is made from the hydrolysis of protein from feathers and/or animal hair such as pig, sheep, or the other poultry feathers and even human hair. Halal status can be doubted (syubhat) and could be also haram. Therefore, the aims of this study were: (1) to isolate L-Cysteine from sheep hair that was sheared while the sheep were still alive (2) to compare the physicochemical properties of L-Cysteine from the sheep with the commercial L-Cysteine, and to compare the quality of white bread with L-Cysteine isolated from fleece, white bread with commercial L-Cysteine and white bread without L-Cysteine added. The results of this study showed that L-Cysteine could be isolated from fleece with a yield of 0.20%; the physicochemical properties of fleece L-Cysteine which comprises shape, isoelectric point, ninhydrin test and Rf value are the same as the physicochemical properties of commercial L-Cysteine, such as form of white crystals: Isoelectric point of 5.07; positive ninhydrin test with the formation of a purple colored complex solution; and the value of Rf is 0.34. The application of the result in the addition of L-Cysteine from fleece in the manufacture of white bread improve the quality of white bread and increasing the panelists preference for the white bread, and the expired date is longer than the expiride date white bread without the addition of L-Cysteine . In other words, L-Cysteine from fleece could be potential as a substitute for non-halal L-Cysteine.
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Anggriawan, I.M.B.2013 Uji aktivitas ekstrak air dan ethanolkulit batang kayumanis padang (Cinnamommum burmanii) dan Jawa (Cinnamommum verum) terhadap enzim α- Glukosidase. Bogor. Program Studi Farmasi Sekolah Tinggi Teknologi Industri dan Farmasi Bogor.
Apriyantono, A. 2008 Mencermati L-Sistein Dalam Bahan Baku Makanan. Republika.co.id [01 Des 2008]
Jandik, P, Pohl, C, Barreto, V, Avdalovic, N. 2001. Anion exchange chromato-graphy and integrated amperometric detection of amino acid. Amino Acid Analysis Protocols. 159 (7): 63-85.
Karlheinz, D. et al. 2007. Amino Acid Ullmann’s Encyclopedia of Industry Chemistry. Doi : 10-1002/14356007 a O 2_057. Pub2. ISBN 978-3-52730673-2.
Jaswir, I., Rahayu, E. A., Yuliana, N. D., Roswiem, A. P., 2020, Daftar Referensi Bahan-Bahan Yang memiliki Titik Kritis Halal Dan Substitusi Bahan non Halal. Jakarta. Komite Nasional Ekonomi dan Keuangan Syariah.
Margriet, R.M.P.I. 2003 Isolasi L-Sistein Dari Bulu Ayam Ras dan Penggunaannya Pada Pembuatan Roti Manis. Serpong. Institut Teknologi Indonesia
McKee, T. and McKee, J.R. 2003. Biochemistry: The Molecular Basis of Life. ed-3. New York. The McGrow Hill Company. Inc
Majelis Ulama Indonesia. 2011. Himpunan Fatwa Majelis ‘Ulama Indonesia Sejak 1975. Jakarta. Sekertariat MUI.
Novita Joseph. 2020. Sistein : Fungsi, Dosis, Efek Samping, Cara Pakai. hellosehat.com [11 Mei 2020]
Roswiem, A. P. 2015. Buku Saku Produk Halal Makanan dan Minuman. Jakarta. Republika Penerbit.
Soekarto, S.T. 1985. Penilaian Organoleptik untuk Industri Pangan dan Hasil Pertanian. Jakarta. Bharata Karya Aksara.