Molecular Detection of Pork Contamination in Beef Sold in Traditional Markets, Yogyakarta
Main Article Content
Abstract
Beef is one of animal protein sources needed by the body to meet nutritional requirements. Consequently, fresh beef (whole or milled) is one of the highest-demanded comestibles on the market. Some meat kiosks in Yogyakarta traditional markets are also on service of milling whole meat. However, there are reports stating that some kiosks are neglecting aspects concerning halal. One of those aspects is separating tools used to process beef and pork. Reports said that kiosk owners fail to meet that basic requirement causing Muslims to be at risk of consuming contaminated beef without their knowledge. Hence, this study aims to examine whether fresh and ground beef obtained from Pasar Pathuk and Pasar Kranggan, Kota Yogyakarta are free from pork contamination. Examination was carried out using Polymerase Chain Reaction (PCR) on 14 samples consisting 7 fresh beef and 7 ground beef obtained from both markets. DNA isolation from all samples were done using FavorGen® FavorPrepTM Tissue Genomic DNA Extraction Mini Kit. Isolated DNA was further examined by PCR analysis using P14 and MTCB primers. Results showed that P14 primers could amplify PRE-1 gene (481 bp) designed as pork molecular marker only on positive control (fresh pork) while MTCB primers could amplify cytochrome b gene (1141 bp) designed as mammal molecular marker on all samples involved in this research. Based on the results, we concluded that both fresh and ground beef sold in Pasar Pathuk and Pasar Kranggan, Yogyakarta are not contaminated by pork DNA.
Article Details
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
References
Fibriana, F., Widianti, T., Retnoningsih, A., dan Susanti. (2012). Deteksi Daging Babi pada Produk Bakso di Pusat Kota Salatiga Menggunakan Teknik Polymerase Chain Reaction. Biosaintifika, 4(2), 106-112.
Kurniama, S.S., Mazieda, M.N., Listyorini, D., dan E. S. S. (2014). Optimization of DNA Isolation and Purification Technique From Chili Pepper (Capsicum annum L.). Seminar Nasional XI Pendidikan Biologi FKIP UNS Biologi, Sains, Lingkungan, dan Pembelajarannya. Pendidikan Biologi FKIP UNS, 11(1), 65-70.
Mustafa, H., I, Rachmawati., dan Y, Udin. (2016). Pengukuran Konsentrasi dan Kemurnian DNA Genom Nyamuk Anopheles barbirostris. Jurnal Vektor Penyakit, 10(1), 7-10.
Mustollah, H. (2016). Analisa Profil Protein Gelatin Sapi dan Gelatin Babi Gummy Vitamin C Menggunakan Metode SDS-Page (Sodium Dodecyl Sulphate Poly Acrilamide Gel Elektrophoresis). Thesis. https://repository.uinjkt.ac.id/. Accessed on 18th of May 2021.
Naidu, A., Fitak, R.R., Munguia-Vega, A., dan Culver, M. (2012). Novel Primer for Complete Mitochondrial Cytochorme b Gene Sequencing in Mammals. Molecular Ecology Resources, Volume 12, 191-196.
Nurhayati, B., dan Darmawati, S. (2017). Biologi Sel dan Molekuler. Kementrian Kesehatan Republik Indonesia.
Pratama, P. (2015). Aplikasi Real-Time PCR untuk Mendeteksi Bakteri Salmonella sp. pada Hasil Perikanan. Thesis. Bogor: Institut Pertanian Bogor.
Redaksikibar, (2013). Pencemaran daging Haram di Yogyakarta. http://kibar-uk.org/2013/02/24/pencemaran-daging-haram-di-yogyakarta-bagian-1/. Accessed on 25th of September 2020.
Setiaputri, A.A., Barokah, G.R., Sahaba, Muh.A.B., Arbajayanti, R.D., Fabella, N., Pertiwi, R.M., Nurilmala, M., Nugraha, R., dan Abdullah, A. (2020). Perbandingan Metode Isolasi DNA Pada Produk Perikanan Segar dan Olahan. Jurnal Pengolahan Hasil Perikanan Indonesia, 23(3), 447-658.
Suparningtyas, J.F., Pramudyawardhani, O.D., Purwoko, D., dan Tajuddin, T. (2018). Analisis filogenetik beberapa klon karet dengan marka AFLP (Amplified Fragment Length Polymorphism). Jurnal Bioteknologi & Biosains Indonesia, 5(1), 8-19.
Susilowati, T. (2019). Deteksi Kontaminan DNA Babi Pada Sampel Penggilingan Daging Di Pasar Surya Kota Surabaya Menggunakan Real-Time Polymerase Chain Reaction. Thesis. https://digilib.uinsby.ac.id. Accessed on 20th of September 2020.
Tilawah, S., Sari, R., dan Apridamayanti, P. (2019). Optimasi Volume Dna Marker Dan Volume Dna Hasil Amplifikasi Gen Tetl Resistensi Antibiotik Tetrasiklin Dari Bakteri Bacillus cereus Pada Pasien Ulkus Diabetik. Jurnal Mahasiswa Farmasi Fakultas Kedokteran Untan, Volume 4. Nomor 1.
Triasih, D., Heryanti, B.R., dan Kridasaksana, D. (2016). Kajian Tentang Perlindungan Hukum Bagi Konsumen Terhadap Produk Makanan Bersertifikat Halal. Jurnal Dinamika Sosial Budaya, 18(2), 214-225